Comparison of one-tube multiplex PCR, automated ribotyping and intergenic spacer (ITS) sequencing for rapid identification of Acinetobacter baumannii
نویسندگان
چکیده
منابع مشابه
One-tube multiplex PCR method for rapid identification of mycobacterium tuberculosis.
A rapid, inexpensive, simple, and accurate multiplex polymerase chain reaction (PCR) was developed in a single tube for identification of Mycobacterium tuberculosis. Assessment of sensitivity and specificity of simple PCR was performed with 116 strains of M. tuberculosis complex (MTC) and 144 strains of nontuberculous mycobacteria (NTM) compared with the biochemical method. Specific amplificati...
متن کاملcomparison of zoe and vitapex for canal treatment of necrotic primary teeth
چکیده ندارد.
15 صفحه اولIdentification of Acinetobacter species and genotyping of Acinetobacter baumannii by multilocus PCR and mass spectrometry.
Members of the genus Acinetobacter are ubiquitous in soil and water and are an important cause of nosocomial infections. A rapid method is needed to genotype Acinetobacter isolates to determine epidemiology and clonality during infectious outbreaks. Multilocus PCR followed by electrospray ionization mass spectrometry (PCR/ESI-MS) is a method that uses the amplicon base compositions to genotype ...
متن کاملComparison of (GTG)5-oligonucleotide and ribosomal intergenic transcribed spacer (ITS)-PCR for molecular typing of Klebsiella isolates.
Molecular typing of Klebsiella species has become important for monitoring dissemination of β-lactamase-producers in hospital environments. The present study was designed to evaluate poly-trinucleotide (GTG)(5)- and rDNA intergenic transcribed spacer (ITS)-PCR fingerprint analysis for typing of Klebsiella pneumoniae and Klebsiella oxytoca isolates. Multiple displacement amplified DNA derived fr...
متن کاملPhenotypic and Genotypic Typing of Multidrug - resistant Acinetobacter Baumannii by Plasmid Profiles and Multiplex - PCR Typing
Profiles and Multiplex -PCR Typing,” Science Journal of Microbiology, Volume 2012, Article ID SJMB-274, 7 Pages, 2012. doi:10.7237/sjmb/274 Abstract–Acinetobacter baumannii, as important opportunistic pathogens, have been found to be responsible for an increasing number of Multiple-drug resistant(MDR) nosocomial infections in worldwide. In the present study, various isolates of MDR, obtained fr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2007
ISSN: 1198-743X
DOI: 10.1111/j.1469-0691.2007.01744.x